To boost cancer discoveries at a cellular level, a new confocal microscope is now
available to Cancer Center Amsterdam researchers. The microscope set-up is
specifically designed to look at subcellular structures in biological samples:
from nuclear division, to mitochondria, or exosome dynamics - to name some imaging possibilities.

The microscope can capture images with a 25 mm field of view which is nearly twice the area of conventional point scanners. This allows significantly more data collection by capturing more of the specimen in each frame. The microscope supports efficient, high-throughput imaging of even large-scale samples by using stitching.

The high-speed imaging capability - up to 720 frames per second - reduces the exposure time to excitation light when live-cell imaging, minimizing phototoxicity and photobleaching.

This microscope was previously installed and successfully used for immunology and cancer research as a part of the Nikon supported Center of Excellence at Amsterdam UMC and was subsequently purchased.

The instrument is located in room CCA 3.07b and training requests can be made via PPMS.

Microscope specifications

Nikon Eclipse Ti2 with a confocal attachment consisting of high-resolution objectives 100x NA 1.45, scan head model A1R HD25, 4 lasers and 4 detectors.

For more information contact: Marko Popovic.

People involved at Cancer Center Amsterdam

Marko Popovic

Michiel Pegtel

Juan J. Garcia Vallejo