After the first basics on RT-PCR, mathematics and statistics, the participants attend lectures and perform hands-on computer exercises on primer design, analysis of amplification curves, using reference genes, removal of systematic between-plate variation and statistical analysis of the final data. In computer hands-on the participants will be introduced to the LinRegPCR analysis application, both the Windows and web-based version. The laboratory exercises include the pipetting of two PCR plates by the participants. The results of this exercise will be analyzed, corrected for between-plate variation and discussed in the final session of the course. A guest lecturer will discuss and demonstrate a new web-based application for analysis of amplification and melting curves data.
The course primarily aims at maximally 24 PhD students who have to register through the website of the Amsterdam UMC Doctoral school.
However, because not all spots may be filled, senior technicians and Post-Docs using qPCR are welcome to express their interest via the course organizer email@example.com. In the week before the course, they will be informed on whether they can participate.